nmol l tumor necrosis factor  (R&D Systems)

Journal: Poultry Science

Article Title: Oral administration of recombinant Lactobacillus plantarum co expressing FimB, CnaA, NetB and FBA antigens targeting chicken dendritic cells provides effective protection against necrotizing enteritis in broilers

doi: 10.1016/j.psj.2026.106889

Figure Lengend Snippet: Production of intracellular cytokines in spleen lymphocytes and intestinal samples. (A) Relative mRNA expression of IFN-γ and IL-4 in splenocytes.(B) Concentrations of IFN-γ and IL-4 in splenocyte culture supernatants. (C) Relative mRNA expression of IFN-γ and IL-4 in intestinal tissues. Data are presented as mean ± SD ( n = 8–10);* P < 0.05 vs. PBS control group and NC8 control group.

Article Snippet: For differentiation, cells were resuspended in complete RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 10 ng/mL recombinant chicken GM-CSF, and 10 ng/mL recombinant chicken IL-4 (KingFisher Biotech).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters

doi: 10.1016/j.psj.2026.107046

Figure Lengend Snippet: SDS-PAGE analysis of expression of pET21a-GnRH6-CRM197 in E. coli. BL21. Lane 1, standard molecular weight marker; Lane 2, non-induced recombinant bacteria; Lane 3, recombinant bacteria induced after 12 h.

Article Snippet: The GnRH6-CRM197 recombinant protein was separated on a 12.5% SDS polyacrylamide gel at 120 V. It was then transferred to a 0.45μm polyvinylidene fluoride (PVDF) membrane (Pulilai, Beijing, China) at 250 mA for 1 hour using a transfer system (BVT-2, Servicebio, Wuhan, China).

Techniques: SDS Page, Expressing, Molecular Weight, Marker, Recombinant, Bacteria

Journal: Poultry Science

Article Title: Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters

doi: 10.1016/j.psj.2026.107046

Figure Lengend Snippet: WB analysis of recombinant GnRH6-CRM197 protein. Lane 1, standard molecular weight marker; Lane 2, recombinant GnRH6-CRM197 fusion protein reacted with rabbit polyclonal to GnRH (dilution 1:2000) .

Article Snippet: The GnRH6-CRM197 recombinant protein was separated on a 12.5% SDS polyacrylamide gel at 120 V. It was then transferred to a 0.45μm polyvinylidene fluoride (PVDF) membrane (Pulilai, Beijing, China) at 250 mA for 1 hour using a transfer system (BVT-2, Servicebio, Wuhan, China).

Techniques: Recombinant, Molecular Weight, Marker

Journal: Poultry Science

Article Title: Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters

doi: 10.1016/j.psj.2026.107046

Figure Lengend Snippet: The effect of recombinant anti-GnRH6-CRM197 on serum anti-GnRH antibody of roosters. The arrow refers to the immune time; the first immunity of roosters is 4 W; * P < 0.05; ** P < 0.01 (n = 20 per group).

Article Snippet: The GnRH6-CRM197 recombinant protein was separated on a 12.5% SDS polyacrylamide gel at 120 V. It was then transferred to a 0.45μm polyvinylidene fluoride (PVDF) membrane (Pulilai, Beijing, China) at 250 mA for 1 hour using a transfer system (BVT-2, Servicebio, Wuhan, China).

Techniques: Recombinant

Journal: Poultry Science

Article Title: Immunocastration with recombinant GnRH6-CRM197 fusion protein improves reproductive suppression and meat tenderness in roosters

doi: 10.1016/j.psj.2026.107046

Figure Lengend Snippet: The effect of recombinant GnRH6-CRM197 on serum testosterone levels of roosters. * P < 0.05; ** P < 0.01 (n = 20 per group).

Article Snippet: The GnRH6-CRM197 recombinant protein was separated on a 12.5% SDS polyacrylamide gel at 120 V. It was then transferred to a 0.45μm polyvinylidene fluoride (PVDF) membrane (Pulilai, Beijing, China) at 250 mA for 1 hour using a transfer system (BVT-2, Servicebio, Wuhan, China).

Techniques: Recombinant